分享产品介绍 评论(0)
宿主来源
Rat抗原名称
4-1BB (CD137)分子别名
Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor; T-cell antigen 4-1BB; Ila; Ly63; Tnfrsf9细胞定位
Cell membraneAccession
P20334克隆号
3H3抗体类型
Recombinant mAb抗体同种型
Rat IgG2a, κ同型对照
Invivo rat IgG2a isotype control, anti-trinitrophenol反应种属 ?
Ms纯化方式
Protein A浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<2EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
in vivo 4-1BB stimulation
in vitro 4-1BB stimulation
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms
4-1BB (CD137, TNFRSF9) is a 39 kDa inducible costimulatory receptor expressed on activated T, NK, NKT and dendritic cells that, upon engagement by its trimeric ligand 4-1BBL or agonistic antibodies, recruits TRAF1/2 to trigger NF-κB, MAPK and PI3K–Akt signaling cascades, thereby enhancing proliferation, survival, cytokine production and cytotoxicity of CD8⁺ and CD4⁺ T cells and NK cells; its expression is tightly regulated (peaking 24–72 h post-activation and restrained by PD-1/CTLA-4 pathways) and its therapeutic agonism is exploited in immuno-oncology to overcome tumor-induced exhaustion, while antagonists are explored for autoimmune diseases.
免疫印迹
WB result of Invivo anti-mouse 4-1BB (CD137) Recombinant mAb
Primary antibody: Invivo anti-mouse 4-1BB (CD137) Recombinant mAb at 1/1000 dilution
Lane 1: 4-1BB/TNFRSF9 His Tag Protein, Mouse 10 ng
Lane 2: 4-1BB/TNFRSF9 His Tag Protein, Rat 2 µg
Lane 3: 4-1BB/TNFRSF9 His Tag Protein, Human 2 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 25-35 kDa
评论(0)