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Rabbit抗原名称
FMC63 scFv免疫原
Recombinant Protein克隆号
S-2163-53抗体类型
Recombinant mAb抗体同种型
IgG纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Species independent
FMC63 scFv (single-chain variable fragment) is a recombinant protein derived from the variable regions (VH and VL) of the anti-CD19 monoclonal antibody FMC63, linked by a flexible peptide spacer (e.g., (Gly₄Ser)₃). It specifically binds to the B-cell surface antigen CD19 with high affinity (KD ~1-10 nM) and is a key targeting component in FDA-approved CAR-T therapies, such as Kymriah®. The humanized FMC63 scFv retains potent antigen recognition while minimizing immunogenicity, making it highly effective in treating B-cell malignancies, including acute lymphoblastic leukemia (ALL) and non-Hodgkin lymphoma (NHL). Ongoing research focuses on optimizing its affinity, stability, and functionality to enhance the safety and efficacy of CAR-T cell therapies.
流式分析
Flow cytometric analysis of FMC63 expression on CAR (FMC63 scFv) transfected Jurkat (human T cell leukemia T lymphocyte). CAR (FMC63 scFv) transfected Jurkat (right panel) or Jurkat (left panel) were labeled with FMC63 antibody at 1/500 dilution (0.1 μg). Goat Anti- Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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