eIF3G Rabbit Polyclonal Antibody
Eukaryotic translation initiation factor 3 subunit G,Eukaryotic translation initiation factor 3 RNA-binding subunit (eIF-3 RNA-binding subunit),Eukaryotic translation initiation factor 3 subunit 4,eIF-3-delta,eIF3 p42,eIF3 p44,EIF3S4 UniRule Annotation,EIF3G
分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
eIF3G分子别名
Eukaryotic translation initiation factor 3 subunit G; Eukaryotic translation initiation factor 3 RNA-binding subunit (eIF-3 RNA-binding subunit); Eukaryotic translation initiation factor 3 subunit 4; eIF-3-delta; eIF3 p42; eIF3 p44; EIF3S4 UniRule Annotation; EIF3G免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
O75821抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Mk阳性样本
Raji, HL-60, K562, HepG2, COS-7纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Mk IHC-P 1:250 Hu
eIF3G, also known as eukaryotic translation initiation factor 3 subunit G or p35, is a crucial component of the eIF3 multi-protein complex, which serves as the largest and most complex initiation factor in eukaryotic translation. Structurally, eIF3G contains multiple RNA recognition motifs (RRMs) that enable it to directly bind specific mRNA sequences and interact with other eIF3 subunits, thereby playing a pivotal role in stabilizing the eIF3 complex and facilitating the assembly of the 43S pre-initiation complex at the 5' cap of messenger RNAs. Beyond its canonical function in promoting the recruitment of the 40S ribosomal subunit to mRNA, eIF3G has been implicated in the selective translation of specific transcripts involved in cell cycle regulation and stress responses, and its dysregulation or overexpression has been frequently observed in various human cancers, suggesting its potential as a biomarker and therapeutic target in oncology due to its influence on protein synthesis rates and cellular proliferation.
免疫印迹
WB result of eIF3G Rabbit pAb
Primary antibody: eIF3G Rabbit pAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: HL-60 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 40 kDaWB result of eIF3G Rabbit pAb
Primary antibody: eIF3G Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 40 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-Eif3g antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Eif3g antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Eif3g antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of eIF3G in tumor tissues.
Expression of eIF3G in human tissues.
评论(0)