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宿主来源
Rabbit抗原名称
CHCHD2分子别名
Coiled-coil-helix-coiled-coil-helix domain-containing protein 2; Aging-associated gene 10 protein; HCV NS2 trans-regulated protein (NS2TP); C7orf17免疫原
Synthetic Peptide细胞定位
Nucleus, MitochondrionAccession
Q9Y6H1克隆号
S-4107-30抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
K562, A431, mouse pancreas, mouse heart, mouse live纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:500-1:2000 Hu, Ms, Rt
CHCHD2 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing Protein 2, also known as MNRR1 or PARK22) is a small nuclear-encoded protein primarily localized in the mitochondrial intermembrane space. It belongs to the protein family containing a conserved CX₉C motif, which folds into a characteristic CHCH domain via disulfide bond formation. This protein plays a central role in maintaining mitochondrial homeostasis: it acts both as a regulator of oxidative phosphorylation (particularly cytochrome c oxidase), helping to stabilize mitochondrial cristae structure, and as a transcriptional coactivator that translocates to the nucleus under cellular stress to regulate the expression of hypoxia-related genes. Additionally, by interacting with BCL-2 family proteins, CHCHD2 functions as a negative regulator of mitochondria-mediated apoptosis, exerting anti-apoptotic effects. On the pathological front, dominant point mutations in the CHCHD2 gene (e.g., T61I) have been confirmed as a causative factor for autosomal dominant Parkinson's disease: the mutations lead to protein misfolding and aggregation within mitochondria, subsequently triggering mitochondrial respiratory chain dysfunction, accumulation of reactive oxygen species, and promoting the abnormal aggregation of α-synuclein, ultimately resulting in the functional impairment of dopaminergic neurons. This gain-of-function toxic mechanism positions CHCHD2 as a key molecular node linking mitochondrial dysfunction to the pathological processes of neurodegenerative diseases, making it a potential target for precision therapy.
免疫印迹
WB result of CHCHD2 Recombinant Rabbit mAb
Primary antibody: CHCHD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 22 kDaWB result of CHCHD2 Recombinant Rabbit mAb
Primary antibody: CHCHD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: mouse pancreas lysate 20 µg
Lane 4: mouse heart lysate 20 µg
Lane 5: mouse liver lysate 20 µg
Low expression control: mouse brain lysate; mouse spleen lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 22 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-CHCHD2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CHCHD2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-CHCHD2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CHCHD2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-CHCHD2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-CHCHD2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CHCHD2 in tumor tissues.
Expression of CHCHD2 in human tissues.
Expression of CHCHD2 in mouse & rat tissues.
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