CD44 Rabbit Polyclonal Antibody
CD44 antigen,CDw44,Epican,Extracellular matrix receptor III (ECMR-III),GP90 lymphocyte homing/adhesion receptor,HUTCH-I,Heparan sulfate proteoglycan,Hermes antigen,Hyaluronate receptor,Phagocytic glycoprotein 1 (PGP-1),Phagocytic glycoprotein I (PGP-I),LHR,MDU2,MDU3,MIC4
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宿主来源
Rabbit抗原名称
CD44分子别名
CD44 antigen; CDw44; Epican; Extracellular matrix receptor III (ECMR-III); GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1 (PGP-1); Phagocytic glycoprotein I (PGP-I); LHR; MDU2; MDU3; MIC4免疫原
Recombinant Protein细胞定位
Secreted, Cell membraneAccession
P16070抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, PANC-1, A549, C2C12, C6, COS-7纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:1000 Hu
CD44 is a multifunctional transmembrane glycoprotein that functions as a major adhesion molecule for hyaluronan and other extracellular matrix components, playing critical roles in cell migration, proliferation, differentiation, and survival; it is expressed in numerous isoforms generated through alternative splicing, with its standard form (CD44s) and variant isoforms (CD44v) exhibiting distinct tissue distributions and functional properties, and its dysregulated expression has been implicated in various pathological processes including inflammation, autoimmune diseases, and cancer progression where it contributes to tumor cell invasion, metastasis, and resistance to apoptosis.
免疫印迹
WB result of CD44 Rabbit pAb
Primary antibody: CD44 Rabbit pAb at 1/1000 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: PANC-1 whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Negative control: LNCaP whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 85 kDaWB result of CD44 Rabbit pAb
Primary antibody: CD44 Rabbit pAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 85 kDaWB result of CD44 Rabbit pAb
Primary antibody: CD44 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 85 kDaWB result of CD44 Rabbit pAb
Primary antibody: CD44 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 85 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-CD44 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD44 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD44 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-CD44 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CD44 in tumor tissues.
Expression of CD44 in human tissues.
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