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宿主来源
Rabbit抗原名称
CAP1分子别名
Adenylyl cyclase-associated protein 1; CAP免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q01518抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
293T, HeLa, MCF7, L-929, mouse lung, rat lung, COS-7纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt, Mk IHC-P 1:1000 Hu
CAP1, also known as Adenylate Cyclase-Associated Protein 1, is a highly conserved and multifunctional actin-regulatory protein that plays a pivotal role in cytoskeletal dynamics by coordinating actin monomer recycling and filament turnover. Structurally, CAP1 functions as a hexameric complex that bridges the activities of ADF/cofilin and profilin; it accelerates the dissociation of cofilin from ADP-actin monomers, facilitates the exchange of ADP for ATP on these monomers, and subsequently delivers the ATP-actin to profilin for efficient re-polymerization at the barbed ends of filaments. Beyond its canonical function in maintaining the global pool of polymerization-competent actin, CAP1 is implicated in various cellular processes including cell migration, endocytosis, and signal transduction pathways involving cyclic AMP, with dysregulation or mutations in the CAP1 gene being associated with developmental defects and immune disorders in humans, thereby highlighting its essential contribution to cellular architecture and physiological homeostasis.
免疫印迹
WB result of CAP1 Rabbit pAb
Primary antibody: CAP1 Rabbit pAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 54 kDaWB result of CAP1 Rabbit pAb
Primary antibody: CAP1 Rabbit pAb at 1/1000 dilution
Lane 1: L-929 whole cell lysate 20 µg
Lane 2: mouse lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 54 kDaWB result of CAP1 Rabbit pAb
Primary antibody: CAP1 Rabbit pAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 54 kDaWB result of CAP1 Rabbit pAb
Primary antibody: CAP1 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 54 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-CAP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CAP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CAP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CAP1 in tumor tissues.
Expression of CAP1 in human tissues.
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