Calpain 1 Recombinant Rabbit mAb (S-3797-41)
Calpain-1 catalytic subunit,Calcium-activated neutral proteinase 1 (CANP 1),Calpain mu-type,Calpain-1 large subunit,Cell proliferation-inducing gene 30 protein,Micromolar-calpain (muCANP),CAPN1,CANPL1
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宿主来源
Rabbit抗原名称
Calpain 1分子别名
Calpain-1 catalytic subunit; Calcium-activated neutral proteinase 1 (CANP 1); Calpain mu-type; Calpain-1 large subunit; Cell proliferation-inducing gene 30 protein; Micromolar-calpain (muCANP); CAPN1; CANPL1免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P07384克隆号
S-4115-48抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
293T, A431, Jurkat, NIH/3T3, mouse brain, C6, rat brain, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:200 Hu, Ms, Rt
Calpain 1 (CAPN1), also known as μ-calpain, is a ubiquitously expressed, calcium-dependent non-lysosomal cysteine protease in humans. It typically exists as a heterodimer consisting of an 80 kDa catalytic large subunit encoded by the CAPN1 gene and a 28 kDa regulatory small subunit encoded by the CAPNS1 gene. Calpain 1 is uniquely activated by micromolar levels of calcium ions, hence its name μ-calpain (micro-calpain), distinguishing it from its homolog, calpain 2, which requires millimolar calcium concentrations for activation. Under physiological conditions, Calpain 1 is localized to various cellular compartments, including the cytoplasm and mitochondria. It performs limited proteolytic cleavage of specific substrates and participates in key processes such as cytoskeletal remodeling, signal transduction, and cell migration. However, under pathological conditions such as ischemia-reperfusion injury and Alzheimer's disease, disruption of intracellular calcium homeostasis can lead to excessive activation of Calpain 1, resulting in aberrant cleavage of various functional proteins (e.g., apoptosis-inducing factor AIF and junctophilin-2 in the heart), thereby mediating cellular damage and dysfunction. Therefore, precise regulation of Calpain 1 is essential for maintaining cellular health and function, making it a potential therapeutic target for various diseases.
免疫印迹
WB result of Calpain 1 Recombinant Rabbit mAb
Primary antibody: Calpain 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 80 kDaWB result of Calpain 1 Recombinant Rabbit mAb
Primary antibody: Calpain 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 80 kDaWB result of Calpain 1 Recombinant Rabbit mAb
Primary antibody: Calpain 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 80 kDaWB result of Calpain 1 Recombinant Rabbit mAb
Primary antibody: Calpain 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 80 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Calpain 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Calpain 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Calpain 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Calpain 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Calpain 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-Calpain 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Calpain 1 in tumor tissues.
Expression of Calpain 1 in human tissues.
Expression of Calpain 1 in mouse & rat tissues.
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