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宿主来源
Rabbit抗原名称
ATP1B1分子别名
Sodium/potassium-transporting ATPase subunit beta-1; ATP1B免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P05026抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Hu, Ms
ATP1B1, also known as the sodium/potassium-transporting ATPase subunit beta-1, is an essential membrane glycoprotein that serves as the beta subunit of the Na⁺/K⁺-ATPase enzyme complex, which is fundamental for maintaining electrochemical gradients across the plasma membrane of most animal cells. This protein plays a critical role in the structural stability and functional maturation of the alpha catalytic subunit, facilitating its correct folding, trafficking to the cell surface, and protection from proteolytic degradation, thereby enabling the active transport of three sodium ions out of the cell and two potassium ions into the cell against their concentration gradients using energy derived from ATP hydrolysis. Beyond its housekeeping function in regulating cell volume, resting membrane potential, and secondary active transport systems, ATP1B1 is increasingly recognized for its involvement in cell adhesion and signal transduction pathways, acting as a modulator in various physiological processes and pathological conditions, including neurological disorders, cardiovascular diseases, and cancer progression, where its expression levels often correlate with tumor suppression or metastatic potential depending on the cellular context.
免疫印迹
WB result of ATP1B1 Rabbit pAb
Primary antibody: ATP1B1 Rabbit pAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 40-55 kDaWB result of ATP1B1 Rabbit pAb
Primary antibody: ATP1B1 Rabbit pAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Lane 3: rat heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 40-55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-ATP1B1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-ATP1B1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ATP1B1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-ATP1B1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ATP1B1 in tumor tissues.
Expression of ATP1B1 in human tissues.
Expression of ATP1B1 in mouse & rat tissues.
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