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Rabbit抗原名称
SDT-04415分子别名
Small ribosomal subunit protein uS11免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P62263抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HepG2, U-2 OS, mouse liver, rat liver预测反应种属
(反应种属缩写表)C.el,Rb纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms, Rt IF 1:500 Ms
Ribosomal Protein S14 (RPS14) is a fundamental component of the small 40S ribosomal subunit in eukaryotic cells, playing a critical role in the structural integrity and functional accuracy of the protein synthesis machinery. Encoded by the RPS14 gene, this highly conserved protein is essential for the proper assembly of the 40S subunit and facilitates key steps in translation initiation and elongation by helping to maintain the correct configuration of the decoding center, thereby ensuring fidelity in mRNA reading and tRNA selection. Beyond its canonical housekeeping function in ribosome biogenesis, RPS14 has gained significant clinical attention due to its location within the commonly deleted region of chromosome 5q (5q-) in myelodysplastic syndromes (MDS); haploinsufficiency of RPS14 in hematopoietic stem cells leads to impaired erythropoiesis and increased apoptosis, directly linking defective ribosome production to the pathophysiology of this specific subtype of bone marrow failure, while also highlighting its emerging roles in extra-ribosomal functions such as the regulation of p53 stability and cellular stress responses.
免疫印迹
WB result of RPS14 Rabbit pAb
Primary antibody: RPS14 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: U-2 OS whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 17 kDaWB result of RPS14 Rabbit pAb
Primary antibody: RPS14 Rabbit pAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 17 kDaWB result of RPS14 Rabbit pAb
Primary antibody: RPS14 Rabbit pAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 17 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-RPS14 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-RPS14 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervix cancer. Anti-RPS14 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-RPS14 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-RPS14 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-RPS14 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse liver. Anti-RPS14 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of RPS14 in tumor tissues.
Expression of RPS14 in human tissues.
Expression of RPS14 in mouse & rat tissues.
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