分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
RPS3分子别名
Small ribosomal subunit protein uS3免疫原
Synthetic Peptide细胞定位
Cytoskeleton, Nucleus, Mitochondrion, CytoplasmAccession
P23396克隆号
S-4121-27抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, K562, HepG2, Jurkat, NIH/3T3, Neuro-2a, PC-12, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu
Ribosomal Protein S3 (RPS3) is a multifunctional component of the 40S small ribosomal subunit in eukaryotes, primarily recognized for its essential role in protein synthesis by facilitating the accurate decoding of mRNA and maintaining translational fidelity through its interaction with the 18S rRNA. Beyond its canonical function in translation, RPS3 exhibits significant "moonlighting" activities, particularly as an extraribosomal protein involved in DNA repair mechanisms, where it acts as a DNA glycosylase to remove oxidized bases such as 8-oxoguanine, thereby protecting genomic integrity against oxidative stress. Furthermore, RPS3 participates in various cellular signaling pathways, including the regulation of apoptosis and the NF-κB pathway, and has been implicated in viral infection processes, such as serving as a receptor or cofactor for certain viruses, highlighting its critical importance in both fundamental cellular homeostasis and disease pathogenesis.
免疫印迹
WB result of RPS3 Recombinant Rabbit mAb
Primary antibody: RPS3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 30 kDaWB result of RPS3 Recombinant Rabbit mAb
Primary antibody: RPS3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 30 kDaWB result of RPS3 Recombinant Rabbit mAb
Primary antibody: RPS3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 30 kDaWB result of RPS3 Recombinant Rabbit mAb
Primary antibody: RPS3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 30 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human brain. Anti-RPS3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-RPS3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse brain. Anti-RPS3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-RPS3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in K562 cells. Anti- RPS3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of RPS3 in tumor tissues.
Expression of RPS3 in human tissues.
Expression of RPS3 in mouse & rat tissues.
评论(0)