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宿主来源
Rabbit抗原名称
HER2/ErbB2分子别名
Receptor tyrosine-protein kinase erbB-2; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; p185erbB2; CD340; Kiaa3023; Neu; Erbb2免疫原
Synthetic Peptide细胞定位
Cell membrane, Cytoplasm, Nucleus, EndosomeAccession
P70424克隆号
S-3935-58抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
4T1预测反应种属
(反应种属缩写表)/纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:2000 Ms, Rt
HER2 (Human Epidermal Growth Factor Receptor 2), also known as ErbB2 or Neu, is a transmembrane tyrosine kinase receptor belonging to the epidermal growth factor receptor (EGFR/ErbB) family, which plays a pivotal role in regulating cell growth, survival, and differentiation through signal transduction pathways such as PI3K/AKT and MAPK. Unlike other members of the ErbB family, HER2 has no known high-affinity ligand and exists in an open conformation ready for dimerization, primarily forming homodimers or heterodimers with other ErbB receptors like EGFR (ErbB1), ErbB3, or ErbB4 upon ligand binding to its partners, thereby amplifying downstream oncogenic signaling. The ERBB2 gene, located on chromosome 17q12, is frequently amplified or overexpressed in various malignancies, most notably in approximately 15–20% of invasive breast cancers, where it correlates with aggressive tumor behavior, increased proliferation, and poorer prognosis; however, this specific molecular profile has become a critical therapeutic target, leading to the development of highly effective targeted therapies including monoclonal antibodies like trastuzumab and pertuzumab, as well as tyrosine kinase inhibitors and antibody-drug conjugates, which have significantly improved clinical outcomes for patients with HER2-positive cancers.
免疫印迹
WB result of HER2/ErbB2 Recombinant Rabbit mAb
Primary antibody: HER2/ErbB2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 4T1 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 139 kDa
Observed MW: 190 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse breast. Anti-HER2/ErbB2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse spleen. Anti-HER2/ErbB2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat breast. Anti-HER2/ErbB2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat heart. Anti-HER2/ErbB2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of HER2/ErbB2 in mouse & rat tissues.
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