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宿主来源
Rabbit抗原名称
FoxO1分子别名
Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FKHR; FOXO1A; FOXO1细胞定位
Cytoplasm, NucleusAccession
Q12778克隆号
S-5118抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
293T, C6, COS-7预测反应种属
(反应种属缩写表)Mk纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms, Rt
FoxO1 (Forkhead box protein O1) is a critical transcription factor belonging to the FoxO subfamily of the forkhead box family, playing a pivotal role in regulating cellular processes such as apoptosis, cell cycle progression, DNA damage repair, oxidative stress resistance, and metabolism, particularly in glucose homeostasis and lipid metabolism. Primarily regulated by the PI3K/Akt signaling pathway, FoxO1 undergoes phosphorylation in response to insulin or growth factors, leading to its exclusion from the nucleus and subsequent inhibition of its transcriptional activity; conversely, under conditions of low insulin or cellular stress, dephosphorylated FoxO1 translocates into the nucleus where it binds to specific DNA sequences to activate target genes like PEPCK and G6Pase, thereby promoting gluconeogenesis in the liver. Beyond its metabolic functions, FoxO1 is essential for maintaining stem cell quiescence, regulating immune responses, and suppressing tumorigenesis, making it a significant molecule of interest in understanding diseases such as type 2 diabetes, cancer, and aging-related disorders.
免疫印迹
WB result of FoxO1 Recombinant Rabbit mAb
Primary antibody: FoxO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HL-60 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Negative control: HL-60 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 68-80 kDaWB result of FoxO1 Recombinant Rabbit mAb
Primary antibody: FoxO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 68-80 kDaWB result of FoxO1 Recombinant Rabbit mAb
Primary antibody: FoxO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 68-80 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-FoxO1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervix cancer. Anti-FoxO1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-FoxO1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-FoxO1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-FoxO1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of FoxO1 in tumor tissues.
Expression of FoxO1 in human tissues.
Expression of FoxO1 in mouse & rat tissues.
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