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宿主来源
Rabbit抗原名称
CDK6分子别名
Cyclin-dependent kinase 6; CR2 protein kinase (CRK2); Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE; Cdkn6; Crk2; Cdk6免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cytoskeleton, NucleusAccession
Q64261克隆号
S-4090-71抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
RAW264.7, mouse testis, mouse spleen, mouse thymus, rat testis, rat spleen, rat thymus预测反应种属
(反应种属缩写表)Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:500 Ms, Rt
Cyclin-dependent kinase 6 (CDK6) is a serine/threonine protein kinase that plays a pivotal role in regulating the cell cycle, specifically during the G1 to S phase transition, by forming active heterodimeric complexes with D-type cyclins (Cyclin D1, D2, or D3). Upon binding to these cyclins, the CDK6 complex phosphorylates the retinoblastoma protein (Rb), which leads to the release of E2F transcription factors and subsequently drives the expression of genes required for DNA synthesis and cell proliferation. Beyond its canonical function in cell cycle control, CDK6 is implicated in various physiological processes including hematopoiesis, differentiation, and metabolism, and its dysregulation—often through gene amplification or overexpression—is frequently observed in numerous cancers, such as breast cancer, lymphoma, and glioblastoma, making it a significant therapeutic target for selective CDK4/6 inhibitors like palbociclib, ribociclib, and abemaciclib, which are widely used in clinical settings to halt tumor growth by inducing G1 cell cycle arrest.
免疫印迹
WB result of CDK6 Recombinant Rabbit mAb
Primary antibody: CDK6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Lane 4: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDaWB result of CDK6 Recombinant Rabbit mAb
Primary antibody: CDK6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Lane 3: rat thymus lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse colon. Anti-CDK6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CDK6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-CDK6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-CDK6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CDK6 in mouse & rat tissues.
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