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Rabbit抗原名称
ARHGAP25分子别名
Rho GTPase-activating protein 25; KIAA0053免疫原
Synthetic PeptideAccession
P42331克隆号
S-4005-5抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Ramos, Raji, Jurkat, Daudi, THP-1纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu IHC-P 1:500 Hu
ARHGAP25, also known as Rho GTPase-activating protein 25, is a critical regulatory protein belonging to the ARHGAP family that functions primarily as a GTPase-activating protein (GAP) for the Rho family of small GTPases, specifically targeting Rac1 and Cdc42 to accelerate their conversion from the active GTP-bound state to the inactive GDP-bound state, thereby negatively regulating cytoskeletal dynamics, cell migration, and phagocytosis. Predominantly expressed in hematopoietic cells, particularly neutrophils and macrophages, ARHGAP25 plays a pivotal role in modulating immune responses by controlling reactive oxygen species (ROS) production via the NADPH oxidase complex and regulating the formation of lamellipodia and filopodia, which are essential for cell motility and pathogen engulfment; furthermore, its activity is tightly regulated through interactions with various signaling molecules and post-translational modifications, ensuring precise spatial and temporal control over Rho GTPase signaling pathways, which, when dysregulated, can contribute to inflammatory diseases, immunodeficiencies, or cancer metastasis, highlighting its significance as a potential therapeutic target in conditions involving aberrant cell movement and immune dysfunction.
免疫印迹
WB result of ARHGAP25 Recombinant Rabbit mAb
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: ARHGAP25 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: Daudi whole cell lysate 20 µg
Lane 6: THP-1 whole cell lysate 20 µg
Negative control: PANC-1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 77 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-ARHGAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-ARHGAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ARHGAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-ARHGAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ARHGAP25 in tumor tissues.
Expression of ARHGAP25 in human tissues.
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