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宿主来源
Rabbit抗原名称
ALCAM分子别名
CD166 antigen; Activated leukocyte cell adhesion molecule; MEMD; CD166免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q13740克隆号
S-4080-21抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
JAR, J774A.1, THP-1, SH-SY5Y, HuT-78, Daudi, mouse brain, mouse liver, rat brain, rat liver预测反应种属
(反应种属缩写表)Bv, Rb纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt
CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM), is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is encoded by the ALCAM gene and is involved in both homotypic and heterotypic cell adhesion. CD166 is expressed on various cell types, including activated T cells, epithelial cells, fibroblasts, and neurons. It plays a crucial role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during T cell development. Additionally, CD166 is overexpressed in several types of tumors and serves as a potential cancer stem cell marker, making it a target for cancer research and therapy.
免疫印迹
WB result of ALCAM Recombinant Rabbit mAb
Primary antibody: ALCAM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: JAR whole cell lysate 20 µg
Lane 2: J774A.1 whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Lane 5: HuT-78 whole cell lysate 20 µg
Lane 6: Daudi whole cell lysate 20 µg
Negative control: JAR whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 100-130 kDaWB result of ALCAM Recombinant Rabbit mAb
Primary antibody: ALCAM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 80-110 kDaWB result of ALCAM Recombinant Rabbit mAb
Primary antibody: ALCAM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 80-110 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human stomach. Anti-ALCAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung. Anti-ALCAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-ALCAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ALCAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate cancer. Anti-ALCAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-ALCAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-ALCAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ALCAM in tumor tissues.
Expression of ALCAM in human tissues.
Expression of ALCAM in mouse & rat tissues.
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