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Rabbit抗原名称
USP14分子别名
Ubiquitin carboxyl-terminal hydrolase 14; Deubiquitinating enzyme 14; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14; TGT免疫原
Synthetic Peptide细胞定位
Cell membrane, CytoplasmAccession
P54578克隆号
S-4022-13抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, Molt-4, SK-OV-3, DU 145, Neuro-2a, L-929, mouse heart, C6, rat heart预测反应种属
(反应种属缩写表)Bv, Cz, Rb纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:800 Hu
USP14 (Ubiquitin Specific Peptidase 14) is a deubiquitinating enzyme that serves as a critical regulatory subunit of the 26S proteasome, functioning primarily at the interface between the 19S regulatory particle and the 20S core particle to modulate protein degradation. By reversibly removing ubiquitin chains from substrate proteins prior to their entry into the proteolytic core, USP14 acts as a "gatekeeper" that can either rescue substrates from degradation or fine-tune the rate of proteolysis, thereby playing a pivotal role in maintaining cellular protein homeostasis and regulating key physiological processes such as DNA damage response, oxidative stress management, and immune signaling. Due to its ability to stabilize oncoproteins and influence tumor cell survival, USP14 has emerged as a significant therapeutic target in cancer research, where its inhibition is being explored to enhance the degradation of disease-causing proteins and sensitize tumors to chemotherapy, highlighting its dual potential as both a protector of cellular integrity and a driver of pathological states when dysregulated.
免疫印迹
WB result of USP14 Recombinant Rabbit mAb
Primary antibody: USP14 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Molt-4 whole cell lysate 20 µg
Lane 3: SK-OV-3 whole cell lysate 20 µg
Lane 4: DU 145 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDaWB result of USP14 Recombinant Rabbit mAb
Primary antibody: USP14 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: L-929 whole cell lysate 20 µg
Lane 3: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDaWB result of USP14 Recombinant Rabbit mAb
Primary antibody: USP14 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDaWB result of USP14 Recombinant Rabbit mAb
Primary antibody: USP14 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-USP14 antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-USP14 antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-USP14 antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of USP14 in tumor tissues.
Expression of USP14 in human tissues.
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