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宿主来源
Rabbit抗原名称
Sec61B分子别名
Protein transport protein Sec61 subunit beta; SEC61B免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulumAccession
P60468抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Mk阳性样本
293T, HeLa, HepG2, A431, C2C12, COS-7预测反应种属
(反应种属缩写表)Dg纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Mk IHC-P 1:1000 Hu
Sec61B is a critical subunit of the Sec61 translocon complex, which serves as the primary protein-conducting channel in the endoplasmic reticulum (ER) membrane of eukaryotic cells. As part of the heterotrimeric Sec61 complex—composed of Sec61α, Sec61β (Sec61B), and Sec61γ—Sec61B plays an essential structural and regulatory role in facilitating the co-translational translocation of nascent polypeptide chains into the ER lumen or their integration into the ER membrane. Although it is the smallest subunit, Sec61B stabilizes the complex by interacting with Sec61α and helps maintain the integrity of the translocation pore, thereby ensuring efficient protein secretion and membrane protein biogenesis. Beyond its canonical role in protein translocation, emerging evidence suggests that Sec61B may also participate in ER stress responses, calcium homeostasis, and the regulation of channel gating, highlighting its multifaceted importance in cellular proteostasis and overall ER function.
免疫印迹
WB result of Sec61B Rabbit pAb
Primary antibody: Sec61B Rabbit pAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 10 kDa
Observed MW: 13 kDaWB result of Sec61B Rabbit pAb
Primary antibody: Sec61B Rabbit pAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 10 kDa
Observed MW: 13 kDaWB result of Sec61B Rabbit pAb
Primary antibody: Sec61B Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 10 kDa
Observed MW: 13 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Sec61B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Sec61B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Sec61B in human tissues.
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