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Rabbit抗原名称
PHD-2分子别名
Egl nine homolog 1; Hypoxia-inducible factor prolyl hydroxylase 2 (HIF-PH2; HIF-prolyl hydroxylase 2; HPH-2); Prolyl hydroxylase domain-containing protein 2 (PHD2); SM-20; C1orf12; EGLN1免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q9GZT9克隆号
S-4111-17抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
SH-SY5Y, HeLa, HepG2, HEK-293, NIH/3T3, mouse brain, C6, rat brain, COS-7预测反应种属
(反应种属缩写表)/纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk ICC 1:50 Hu
Prolyl hydroxylase domain-containing protein 2 (PHD2), also known as EGLN1, is a crucial iron- and oxygen-dependent dioxygenase that serves as the primary cellular oxygen sensor regulating the hypoxia-inducible factor (HIF) pathway under normoxic conditions. By catalyzing the hydroxylation of specific proline residues on the alpha subunits of HIF, PHD2 marks these transcription factors for von Hippel-Lindau (VHL)-mediated ubiquitination and subsequent proteasomal degradation, thereby preventing the accumulation of HIF and the activation of hypoxia-responsive genes when oxygen levels are sufficient. This enzymatic activity is tightly coupled to cellular metabolic status, as it requires molecular oxygen, ferrous iron, and 2-oxoglutarate as cofactors, making PHD2 a central node in integrating oxygen availability with metabolic signaling; consequently, its dysfunction or inhibition leads to HIF stabilization, which drives adaptive responses such as angiogenesis, erythropoiesis, and metabolic reprogramming, highlighting its significant potential as a therapeutic target for conditions ranging from anemia and ischemic diseases to cancer.
免疫印迹
WB result of PHD-2 Recombinant Rabbit mAb
Primary antibody: PHD-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDaWB result of PHD-2 Recombinant Rabbit mAb
Primary antibody: PHD-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDaWB result of PHD-2 Recombinant Rabbit mAb
Primary antibody: PHD-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDaWB result of PHD-2 Recombinant Rabbit mAb
Primary antibody: PHD-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDa
免疫细胞化学
ICC shows positive staining in SH-SY5Y cells. Anti-PHD-2 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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