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pan SCN Recombinant Rabbit mAb (S-5038)

Sodium channel protein type 2 subunit alpha,HBSC II,Sodium channel protein brain II subunit alpha,Sodium channel protein type II subunit alpha,Voltage-gated sodium channel subunit alpha Nav1.2,SCN2A,HBA,NAC2,SCN2A1,SCN2A2,Sodium channel protein type 1 subunit alpha,Sodium channel protein brain I subunit alpha,Sodium channel protein type I subunit alpha,Voltage-gated sodium channel subunit alpha Nav1.1,SCN1A,NAC1,SCN1,Sodium channel protein type 3 subunit alpha,Sodium channel protein brain III subunit alpha,Sodium channel protein type III subunit alpha,Voltage-gated sodium channel subtype III,Voltage-gated sodium channel subunit alpha Nav1.3,SCN3A,KIAA1356,NAC3,Sodium channel protein type 9 subunit alpha,Neuroendocrine sodium channel (hNE-Na),Peripheral sodium channel 1 (PN1),Sodium channel protein type IX subunit alpha,Voltage-gated sodium channel subunit alpha Nav1.7,SCN9A,NENA

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6943
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    SCN

  • 分子别名

    Sodium channel protein type 2 subunit alpha; HBSC II; Sodium channel protein brain II subunit alpha; Sodium channel protein type II subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.2; SCN2A; HBA; NAC2; SCN2A1; SCN2A2; Sodium channel protein type 1 subunit alpha; Sodium channel protein brain I subunit alpha; Sodium channel protein type I subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.1; SCN1A; NAC1; SCN1; Sodium channel protein type 3 subunit alpha; Sodium channel protein brain III subunit alpha; Sodium channel protein type III subunit alpha; Voltage-gated sodium channel subtype III; Voltage-gated sodium channel subunit alpha Nav1.3; SCN3A; KIAA1356; NAC3; Sodium channel protein type 9 subunit alpha; Neuroendocrine sodium channel (hNE-Na); Peripheral sodium channel 1 (PN1); Sodium channel protein type IX subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.7; SCN9A; NENA

  • 细胞定位

    Cell membrane

  • Accession

    Q99250, P35498, Q9NY46, Q15858

  • 克隆号

    S-5038

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    mouse hippocampus, mouse brain, rat hippocampus, rat brain

  • 预测反应种属
    (反应种属缩写表)

    /

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    WB

    IF ?

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:2000 Ms, Rt
    IHC-P 1:250-1:500 Hu, Ms, Rt
    IF 1:500 Ms, Rt
背景介绍

  • The α subunits of voltage-gated sodium channels are encoded by the SCN gene family (including SCN1A to SCN11A), and constitute the core pore-forming component of these channels. These α subunits share a highly conserved structure, typically consisting of four homologous domains (I-IV) that assemble to form a central pore. Each domain contains six transmembrane helices (S1-S6), with the S4 segment—rich in positively charged amino acid residues—acting as the voltage sensor to detect changes in membrane potential. Upon membrane depolarization, the outward movement of the S4 segment triggers rapid channel activation and opening, allowing a sodium influx that generates the upstroke of the action potential. The channel then rapidly inactivates within milliseconds. The distribution and function of different SCN family members are highly specialized: SCN1A, SCN2A, and SCN3A are predominantly expressed in neurons, where mutations in SCN1A are closely associated with Dravet syndrome (a severe epileptic encephalopathy); SCN4A is mainly found in skeletal muscle, where its mutations can lead to hyperkalemic or hypokalemic periodic paralysis; SCN5A is the primary sodium channel α subunit in cardiomyocytes, playing a critical role in the upstroke (phase 0) of the cardiac action potential and impulse conduction, with pathogenic mutations causing various inherited arrhythmias such as Brugada syndrome and long QT syndrome (type 3). Additionally, SCN9A, SCN10A, and SCN11A are primarily expressed in peripheral sensory neurons and are involved in pain perception. Gain-of-function mutations in SCN9A can cause primary erythermalgia (a painful syndrome), while loss-of-function mutations lead to congenital insensitivity to pain. Because these channels are fundamental to electrical signaling, they serve as important targets for antiepileptic drugs, local anesthetics, and antiarrhythmic agents; consequently, genetic variants in family members can readily lead to severe neurological, muscular, or cardiac disorders.

  • 免疫印迹

    • WB result of pan SCN Recombinant Rabbit mAb
      Primary antibody: pan SCN Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse heart lysate 20 µg
      Lane 2: mouse hippocampus lysate 20 µg
      Lane 3: mouse brain lysate 20 µg
      Negative control: mouse heart lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 228 kDa
      Observed MW: 350 kDa

    • WB result of pan SCN Recombinant Rabbit mAb
      Primary antibody: pan SCN Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat heart lysate 20 µg
      Lane 2: rat hippocampus lysate 20 µg
      Lane 3: rat brain lysate 20 µg
      Negative control: rat heart lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 228 kDa
      Observed MW: 350 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human brain. Anti-pan SCN antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon. Anti-pan SCN antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-pan SCN antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human lung cancer. Anti-pan SCN antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human heart. Anti-pan SCN antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse brain. Anti-pan SCN antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded mouse kidney. Anti-pan SCN antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat brain. Anti-pan SCN antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded rat testis. Anti-pan SCN antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded mouse cerebellum. Anti-pan SCN antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded mouse brain. Anti-pan SCN antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • Negative control: IF shows negative staining in paraffin-embedded mouse cardiac muscle. Anti-pan SCN antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded rat cerebellum. Anti-pan SCN antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded rat brain. Anti-pan SCN antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • Negative control: IF shows negative staining in paraffin-embedded rat cardiac muscle. Anti-pan SCN antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • 组织表达图谱

    • Expression of pan SCN in tumor tissues.

    • Expression of pan SCN in human tissues.

    • Expression of pan SCN in mouse & rat tissues.

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