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宿主来源
Rabbit抗原名称
pan PDE4分子别名
3',5'-cyclic-AMP phosphodiesterase 4D; DPDE3; PDE43; cAMP-specific phosphodiesterase 4D; PDE4D细胞定位
Cell membrane, Cytoplasm, CytoskeletonAccession
Q08499克隆号
S-5034抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, mouse brain, mouse cerebellum, rat brain, rat cerebellum纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Hu, Ms, Rt
PDE4 is a member of the phosphodiesterase 4 family, belonging to the 3',5'-cyclic nucleotide-specific phosphodiesterases. Its primary function is to specifically hydrolyze the second messenger cyclic AMP (cAMP), converting it into inactive 5'-AMP, thereby terminating cAMP-mediated cell signaling pathways. The PDE4 family comprises four subtypes (PDE4A, PDE4B, PDE4C, PDE4D), each generating multiple variants through alternative splicing, with N-terminal targeting domains determining their subcellular localization and interactions with signaling complexes. As an important negative regulator, PDE4 controls local cAMP concentrations, thereby regulating downstream protein kinase A (PKA) and Epac signaling pathways, which in turn affect processes such as cell contraction, migration, proliferation, inflammatory responses, and learning and memory. Due to its high expression in immune and inflammatory cells, PDE4 has become an important therapeutic target for chronic inflammatory diseases, with selective inhibitors (e.g., apremilast, roflumilast) developed for the treatment of psoriasis, ankylosing spondylitis, and chronic obstructive pulmonary disease (COPD). Additionally, PDE4 dysfunction is associated with diseases such as depression, Alzheimer's disease, and asthma, and its inhibitors have shown potential value in neuroprotection and antidepressant effects.
免疫印迹
WB result of pan PDE4 Recombinant Rabbit mAb
Primary antibody: pan PDE4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Low expression control: K562 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 91 kDa
Observed MW: 60-100 kDaWB result of pan PDE4 Recombinant Rabbit mAb
Primary antibody: pan PDE4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse cerebellum lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 91 kDa
Observed MW: 60-100 kDaWB result of pan PDE4 Recombinant Rabbit mAb
Primary antibody: pan PDE4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat cerebellum lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 91 kDa
Observed MW: 60-100 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse brain. Anti-pan PDE4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain. Anti-pan PDE4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human brain. Anti-pan PDE4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of pan PDE4 in tumor tissues.
Expression of pan PDE4 in human tissues.
Expression of pan PDE4 in mouse & rat tissues.
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