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宿主来源
Rabbit抗原名称
Lhx2/LH2分子别名
LIM/homeobox protein Lhx2; Homeobox protein LH-2; LIM homeobox protein 2免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q9Z0S2克隆号
S-3867-28抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse E14 fetal brain, rat E14 fetal brain预测反应种属
(反应种属缩写表)/纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:4000 Ms, Rt IF 1:500 Ms
Lhx2, also known as LIM-homeobox 2 or LH2, is a critical transcription factor belonging to the LIM-homeodomain family that plays indispensable roles in embryonic development and tissue maintenance, particularly within the central nervous system, eye, and liver. Characterized by two N-terminal LIM domains that mediate protein-pro interactions and a C-terminal homeodomain responsible for DNA binding, Lhx2 functions primarily as a nuclear regulator of gene expression, often acting in concert with cofactors such as Ldb1 to modulate chromatin accessibility and transcriptional activity. In the developing brain, it is essential for the specification of cortical identity, hippocampal formation, and the differentiation of olfactory sensory neurons, while in the eye, it governs optic cup morphogenesis and retinal progenitor cell proliferation; furthermore, recent studies have highlighted its significance in adult neurogenesis and its potential involvement in pathological conditions, including its tumor-suppressive or oncogenic duality depending on the cellular context, thereby establishing Lhx2 as a multifaceted regulator crucial for both organogenesis and cellular homeostasis.
免疫印迹
WB result of Lhx2/LH2 Recombinant Rabbit mAb
Primary antibody: Lhx2/LH2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse E14 fetal brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 50 kDaWB result of Lhx2/LH2 Recombinant Rabbit mAb
Primary antibody: Lhx2/LH2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat E14 fetal brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse E14 embryo. Anti-Lhx2/LH2 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded nose skin of E14 mouse embryo. Anti-Lhx2/LH2 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat E14 embryo. Anti-Lhx2/LH2 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded olfactory epithelium tissue of E14 rat embryo. Anti-Lhx2/LH2 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded E14 mouse embryo. Anti- Lhx2/LH2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Lhx2/LH2 in mouse & rat tissues.
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