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Rabbit抗原名称
KLHL21分子别名
Kelch-like protein 21; KIAA0469免疫原
Synthetic Peptide细胞定位
Cytoskeleton, CytoplasmAccession
Q9UJP4克隆号
S-3460-252抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
A431, HeLa, A549, U-87 MG, BxPC-3, Raji, HepG2, MCF7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu IHC-P 1:500 Hu
KLHL21 (Kelch-like family member 21) is a substrate-specific adaptor protein for the Cullin 3-based E3 ubiquitin ligase complex, playing a pivotal role in regulating cellular processes through targeted protein ubiquitination and subsequent degradation. Characterized by its distinctive domain architecture, which includes an N-terminal BTB/POZ domain responsible for binding to Cullin 3 and a C-terminal kelch repeat domain that mediates specific substrate recognition, KLHL21 functions as a critical bridge between the ubiquitination machinery and its target proteins. It is particularly renowned for its essential function in mitosis, where it localizes to chromosomes and the central spindle to regulate the Aurora B kinase pathway, thereby ensuring accurate chromosome segregation and cytokinesis; dysregulation or mutation of KLHL21 has been implicated in various pathological conditions, including neurodevelopmental disorders and certain cancers, highlighting its significance in maintaining genomic stability and proper cell division.
免疫印迹
WB result of KLHL21 Recombinant Rabbit mAb
Primary antibody: KLHL21 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Lane 5: BxPC-3 whole cell lysate 20 µg
Lane 6: Raji whole cell lysate 20 µg
Lane 7: HepG2 whole cell lysate 20 µg
Lane 8: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 67 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-KLHL21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-KLHL21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreas cancer. Anti-KLHL21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of KLHL21 in tumor tissues.
Expression of KLHL21 in human tissues.
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