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Rabbit抗原名称
IL-17A分子别名
Interleukin-17A; IL-17; Cytotoxic T-lymphocyte-associated antigen 8 (CTLA-8); CTLA8; IL17; IL17A免疫原
Recombinant Protein细胞定位
SecretedAccession
Q16552克隆号
S-3055-61抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu
IL-17A is a 155-amino-acid, disulfide-linked homodimeric pro-inflammatory cytokine secreted primarily by Th17 cells, γδ T cells and innate lymphoid cells; it engages the ubiquitously expressed IL-17RA/RC receptor complex to trigger TRAF6- and Act1-dependent signaling cascades that activate NF-κB, MAPKs and C/EBP transcription factors, thereby inducing massive release of neutrophil-mobilizing chemokines (CXCL1, CXCL8), G-CSF, IL-6 and antimicrobial peptides, orchestrating granulopoiesis, extracellular-matrix remodeling and barrier immunity; dysregulated IL-17A underlies autoimmune pathologies such as psoriasis, ankylosing spondylitis and multiple sclerosis, making it the target of FDA-approved biologics like secukinumab and ixekizumab.
免疫印迹
WB result of pan IL-17A Recombinant Rabbit mAb
Primary antibody: IL-17A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293F transfected with empty vector whole cell lysate 20 µg
Lane 2: 293F transfected with IL-17A-Myc-His fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17, 23 kDaWB result of pan IL-17A Recombinant Rabbit mAb
Primary antibody: IL-17A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: IL-17A protein 10 ng
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17, 23 kDa
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