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Rabbit抗原名称
HO-1分子别名
Heme oxygenase 1; HO-1Cleaved into 1 chainsHeme oxygenase 1 soluble form; HO; HO1; HMOX1免疫原
Recombinant Protein细胞定位
Endoplasmic reticulumAccession
P09601抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HeLa (treated with 50 µM arsenite for 8 hours), NIH/3T3 (treated with 50 µM arsenite for 8 hours)纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:1000 Hu, Ms
Heme Oxygenase-1 (HO-1, HSP32) is a 32 kDa stress-responsive enzyme anchored to the smooth endoplasmic reticulum that cleaves heme at the α-meso carbon to release equimolar biliverdin (rapidly reduced to bilirubin), carbon monoxide (CO) and ferrous iron, thereby not only eliminating the pro-oxidant heme but also generating potent antioxidants and the signaling gas CO; transcription of the HMOX1 gene is potently induced by myriad stressors—including heme itself, ROS, heavy metals, cytokines, hypoxia, nitric oxide, and natural electrophiles—via Nrf2/ARE, Bach1, HIF-1, AP-1 and NF-κB pathways, giving HO-1 a central cytoprotective role against oxidative and inflammatory injury in cardiovascular, neuronal, renal and hepatic tissues, modulating mitochondrial biogenesis, autophagy and immune tolerance, while its products bilirubin and CO respectively scavenge radicals and activate cGMP/PKG signaling to vasodilate, inhibit platelet aggregation and suppress apoptosis, making HO-1 a therapeutic target in sepsis, ischemia–reperfusion, transplant rejection, metabolic disorders and neurodegeneration.
免疫印迹
WB result of HO-1 Rabbit pAb
Primary antibody: HO-1 Rabbit pAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 50 µM arsenite for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDaWB result of HO-1 Rabbit pAb
Primary antibody: HO-1 Rabbit pAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 50 µM arsenite for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-HO-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung. Anti-HO-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-HO-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-HO-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-HO-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of HO-1 in tumor tissues.
Expression of HO-1 in human tissues.
Expression of HO-1 in mouse & rat tissues.
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