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宿主来源
Rabbit抗原名称
DGCR8分子别名
Microprocessor complex subunit DGCR8; C22orf12; DGCRK6免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q8WYQ5克隆号
S-4082-6抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, Jurkat, HCT-116, HEK-293, A549, PC-3, Neuro-2a, NIH/3T3, mouse brain, PC-12, rat brain, rat testis纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt
DGCR8 (DiGeorge Syndrome Critical Region 8), also known as Pasha in invertebrates, is an essential double-stranded RNA-binding protein that functions as a core component of the Microprocessor complex alongside the RNase III enzyme Drosha, playing a pivotal role in the canonical biogenesis of microRNAs (miRNAs) within the nucleus. By recognizing and binding to the apical loop and single-stranded RNA segments of primary miRNA transcripts (pri-miRNAs), DGCR8 serves as a molecular ruler that positions Drosha to precisely cleave the pri-miRNA base, thereby generating precursor miRNAs (pre-miRNAs) that are subsequently exported to the cytoplasm for further processing into mature miRNAs involved in post-transcriptional gene regulation. Beyond its central function in miRNA processing, DGCR8 contributes to the maintenance of genomic stability, DNA damage response, and cellular differentiation, and its haploinsufficiency due to deletions in chromosome 22q11.2 is directly linked to DiGeorge syndrome (22q11.2 deletion syndrome), a multisystem developmental disorder characterized by congenital heart defects, immune deficiencies, and neuropsychiatric abnormalities, underscoring the critical importance of precise DGCR8-mediated regulatory networks in human development and disease.
免疫印迹
WB result of DGCR8 Recombinant Rabbit mAb
Primary antibody: DGCR8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Lane 5: A549 whole cell lysate 20 µg
Lane 6: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 100 kDaWB result of DGCR8 Recombinant Rabbit mAb
Primary antibody: DGCR8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 100 kDaWB result of DGCR8 Recombinant Rabbit mAb
Primary antibody: DGCR8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 100 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-DGCR8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-DGCR8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-DGCR8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-DGCR8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-DGCR8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-DGCR8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of DGCR8 in tumor tissues.
Expression of DGCR8 in human tissues.
Expression of DGCR8 in mouse & rat tissues.
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