分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
CEP55分子别名
Centrosomal protein of 55 kDa; C10orf3; URCC6免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
Q53EZ4克隆号
S-3562-33抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
LNCaP, HT-1080, SK-MEL-28, MDA-MB-231, T-47D, MCF7, HeLa, Jurkat, HEK-293, HepG2, mouse testis, rat testis预测反应种属
(反应种属缩写表)/纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt
CEP55 (Centrosomal Protein 55), also known as FLJ10540 or C5orf38, is a coiled-coil protein primarily localized to the midbody during the final stages of cell division, where it plays a critical role in cytokinesis and abscission by recruiting essential machinery such as the ESCRT-III complex and ALIX to ensure the proper physical separation of daughter cells. Beyond its canonical function in mitosis, CEP55 is significantly overexpressed in various malignant tumors, including breast, lung, and liver cancers, acting as an oncogene that promotes cell proliferation, migration, and invasion while inhibiting apoptosis, often through interactions with key signaling pathways like PI3K/AKT and MAPK. Due to its restricted expression in normal adult tissues—being largely absent except in testis—and its high prevalence in cancer cells, CEP55 has emerged as a promising tumor-specific antigen for immunotherapy and a potential prognostic biomarker, linking defective cytokinesis directly to genomic instability and tumorigenesis.
免疫印迹
WB result of CEP55 Recombinant Rabbit mAb
Primary antibody: CEP55 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Lane 2: HT-1080 whole cell lysate 20 µg
Lane 3: SK-MEL-28 whole cell lysate 20 µg
Lane 4: MDA-MB-231 whole cell lysate 20 µg
Lane 5: T-47D whole cell lysate 20 µg
Lane 6: MCF7 whole cell lysate 20 µg
Lane 7: HeLa whole cell lysate 20 µg
Lane 8: Jurkat whole cell lysate 20 µg
Lane 9: HEK-293 whole cell lysate 20 µg
Lane 10: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 55 kDaWB result of CEP55 Recombinant Rabbit mAb
Primary antibody: CEP55 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50 kDaWB result of CEP55 Recombinant Rabbit mAb
Primary antibody: CEP55 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50 kDa
评论(0)