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宿主来源
Rabbit抗原名称
Alix分子别名
Programmed cell death 6-interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95; AIP1; ALIX; KIAA1375; PDCD6IP免疫原
Recombinant Protein细胞定位
Melanosome, Secreted, CytoplasmAccession
Q8WUM4抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, A549, PC-3, MCF7, NIH/3T3, mouse brain, C6, rat brain, COS-7预测反应种属
(反应种属缩写表)/纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk
Alix, also known as ALG-2-interacting protein X or PDCD6IP, is a crucial multifunctional adaptor protein belonging to the Bro1 domain-containing family that plays a pivotal role in regulating endosomal sorting and membrane trafficking processes, particularly within the Endosomal Sorting Complex Required for Transport (ESCRT) pathway. Structurally characterized by an N-terminal Bro1 domain, a central proline-rich region, and a C-terminal V domain, Alix serves as a molecular bridge that interacts with various ESCRT-III components, such as CHMP4, and ubiquitinated cargo proteins to facilitate the formation of intraluminal vesicles in multivesicular bodies, thereby directing cellular proteins toward lysosomal degradation or exosomal release. Beyond its canonical function in membrane remodeling and cytokinesis, Alix is extensively involved in apoptotic signaling through its interaction with apoptosis-linked gene 2 (ALG-2) and participates in viral budding mechanisms, making it a significant subject of study in understanding cellular homeostasis, neurodegenerative diseases, and cancer progression.
免疫印迹
WB result of Alix Rabbit pAb
Primary antibody: Alix Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 90-100 kDaWB result of Alix Rabbit pAb
Primary antibody: Alix Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 90-100 kDaWB result of Alix Rabbit pAb
Primary antibody: Alix Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 90-100 kDaWB result of Alix Rabbit pAb
Primary antibody: Alix Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 90-100 kDa
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