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Rabbit抗原名称
Phospho-S6 Ribosomal Protein (Ser240/244)分子别名
Small ribosomal subunit protein eS6; 40S ribosomal protein S6; Phosphoprotein NP33; RPS6细胞定位
Cytoplasm, NucleusAccession
P62753克隆号
S-4983抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk ICC 1:500 Hu
免疫印迹
WB result of Phospho-S6 Ribosomal Protein (Ser240/244) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-S6 Ribosomal Protein (Ser240/244) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated PC-12 whole cell lysate 20 µg
Lane 2: PC-12 starve overnight, then grown in 20% FBS media for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 32 kDa
免疫细胞化学
ICC shows positive staining in HeLa cells treated with insulin (100 nM, 15 mins) (top panel) and negative staining in HeLa cells treated with LY294002 (50 μM, 2 hr) (below panel). Anti-Phospho-S6 Ribosomal Protein (Ser240/244 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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