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宿主来源
Rabbit抗原名称
Nestin分子别名
Nes免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
Q6P5H2克隆号
S-3890-61抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
IF ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:2000 Ms, Rt IF 1:2000 Ms, Rt
Nestin is a cytoskeletal component belonging to class VI intermediate filaments, initially identified as a marker of neuroepithelial stem cells and neural progenitor cells. It is structurally and expressively unique, featuring a short N-terminus and an extremely long C-terminal domain. Its expression is developmentally regulated: it is highly expressed in proliferating, undifferentiated progenitor cells and rapidly downregulated upon terminal differentiation, where it is largely replaced by other intermediate filament proteins (such as neurofilaments). Beyond the nervous system, Nestin is broadly expressed in local stem/progenitor cells in various tissues including skeletal muscle, heart, pancreas, liver, and hair follicles. As a cytoskeletal protein, Nestin typically co-polymerizes with other intermediate filament proteins (such as vimentin) to form heteropolymeric intermediate filament networks, participating in maintaining cell morphology, regulating cell migration, proliferation, and differentiation. Due to its aberrant high expression in various tumors (such as glioblastoma, melanoma, pancreatic cancer, and prostate cancer), which is closely associated with tumor malignancy, invasiveness, and poor prognosis, Nestin has been widely used as a reliable marker and a potential therapeutic target for cancer stem cells in solid tumors including gliomas.
免疫组化
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Nestin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse brain. Anti-Nestin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse heart. Anti-Nestin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Nestin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Nestin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse kidney. Anti-Nestin antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat kidney. Anti-Nestin antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Nestin in mouse & rat tissues.
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