KDM2A Recombinant Rabbit mAb (S-3943-25)
Lysine-specific demethylase 2A,F-box and leucine-rich repeat protein 11,F-box/LRR-repeat protein 11,JmjC domain-containing histone demethylation protein 1A,[Histone-H3]-lysine-36 demethylase 1A,Fbl11,Fbxl11,Jhdm1a,Kiaa1004,Kdm2a
分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
KDM2A分子别名
Lysine-specific demethylase 2A; F-box and leucine-rich repeat protein 11; F-box/LRR-repeat protein 11; JmjC domain-containing histone demethylation protein 1A; [Histone-H3]-lysine-36 demethylase 1A; Fbl11; Fbxl11; Jhdm1a; Kiaa1004; Kdm2a免疫原
Synthetic Peptide细胞定位
NucleusAccession
P59997克隆号
S-3943-25抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
NIH/3T3, RAW264.7, mouse testis, PC-12, C6纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Ms, Rt IHC-P 1:100 Ms, Rt
KDM2A (also known as FBXL11 or JHDM1A) is a multifunctional protein that primarily functions as a histone H3 lysine 36 (H3K36) demethylase through its JmjC domain, specifically removing H3K36me1/2 marks. As a key epigenetic regulator, it binds specifically to unmethylated CpG islands in the genome via its CXXC zinc finger domain, thereby influencing chromatin structure and gene expression. Additionally, KDM2A contains an F-box domain that enables it to act as a substrate recognition subunit of the SCF (SKP1-Cullin-F-box) E3 ubiquitin ligase complex, participating in protein ubiquitination and degradation. Through its enzymatic activity and protein interactions, KDM2A is widely involved in regulating critical biological processes such as cell proliferation, differentiation, DNA damage repair, and maintenance of genomic stability. It also plays complex, and sometimes contradictory, regulatory roles in various cancers, including non-small cell lung cancer and breast cancer.
免疫印迹
WB result of KDM2A Recombinant Rabbit mAb
Primary antibody: KDM2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 133 kDa
Observed MW: 130 kDa
This blot was developed with high sensitivity substrateWB result of KDM2A Recombinant Rabbit mAb
Primary antibody: KDM2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 133 kDa
Observed MW: 130 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded mouse brain. Anti-KDM2A antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-KDM2A antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain. Anti-KDM2A antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of KDM2A in mouse & rat tissues.
评论(0)