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Rabbit抗原名称
ATP citrate lyase分子别名
ATP-citrate synthase; ATP-citrate (pro-S-)-lyase (ACL); Citrate cleavage enzyme; ACLY细胞定位
CytoplasmAccession
P53396克隆号
S-5036抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, NIH/3T3, mouse lung, C6, rat lung, COS-7预测反应种属
(反应种属缩写表)/纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:2000 Hu, Ms, Rt ICC 1:500 Hu
ACLY, also known as ATP citrate lyase, is a crucial enzyme involved in cellular metabolism. It catalyzes the conversion of citrate and ATP to oxaloacetate, acetyl-CoA, and ADP, playing a key role in linking glycolysis and lipid metabolism. ACLY is predominantly located in the cytoplasm and is essential for the production of acetyl-CoA, which serves as a vital precursor for fatty acid synthesis and cholesterol biosynthesis. Its activity is tightly regulated by various factors, including insulin and AMPK, reflecting its importance in maintaining metabolic homeostasis. Abnormal expression or activity of ACLY has been implicated in several diseases, such as cancer and metabolic disorders, making it an attractive target for therapeutic interventions.
免疫印迹
WB result of ATP Citrate Lyase Recombinant Rabbit mAb
Primary antibody: ATP Citrate Lyase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 121 kDaWB result of ATP Citrate Lyase Recombinant Rabbit mAb
Primary antibody: ATP Citrate Lyase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 121 kDaWB result of ATP Citrate Lyase Recombinant Rabbit mAb
Primary antibody: ATP Citrate Lyase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 121 kDaWB result of ATP Citrate Lyase Recombinant Rabbit mAb
Primary antibody: ATP Citrate Lyase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 121 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human heart. Anti- ATP citrate lyase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti- ATP citrate lyase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal cell carcinoma. Anti- ATP citrate lyase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti- ATP citrate lyase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti- ATP citrate lyase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- ATP Citrate Lyase antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of ATP citrate lyase in tumor tissues.
Expression of ATP citrate lyase in human tissues.
Expression of ATP citrate lyase in mouse & rat tissues.
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