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宿主来源
Rabbit抗原名称
4-1BB/CD137/TNFRSF9分子别名
Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA; ILA细胞定位
Cell membraneAccession
Q07011克隆号
S-4989抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, Jurkat, HDLM-2纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:100 Hu
CD137, also known as 4-1BB, is a co-stimulatory molecule expressed on the surface of activated T cells and natural killer cells. It plays a crucial role in enhancing the immune response by providing additional signals that promote T cell proliferation, survival, and cytokine production. CD137 is a member of the tumor necrosis factor receptor superfamily and its ligand, CD137L, is primarily found on antigen-presenting cells. The interaction between CD137 and CD137L helps to sustain the activation of T cells and supports the development of long-lasting immune responses, making it an important target for immunotherapy in cancer treatment and other diseases where modulation of the immune system is beneficial.
免疫印迹
WB result of 4-1BB/CD137/TNFRSF9 Recombinant Rabbit mAb
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: 4-1BB/CD137/TNFRSF9 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HDLM-2 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate; Jurkat whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 32, 38 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human Hodgkin lymphoma. Anti-4-1BB/CD137/TNFRSF9 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thymus. Anti-4-1BB/CD137/TNFRSF9 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-4-1BB/CD137/TNFRSF9 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of 4-1BB/CD137/TNFRSF9 in tumor tissues.
Expression of 4-1BB/CD137/TNFRSF9 in human tissues.
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