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宿主来源
Mouse抗原名称
Beta Actin分子别名
Actin, cytoplasmic 1; ACTB细胞定位
Cytoskeleton, Nucleus, CytoplasmAccession
P60709克隆号
S-5033抗体类型
Mouse mAb抗体同种型
IgG2b反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, HEK-293, HepG2, K562, Jurkat, NIH/3T3, 4T1, mouse brain, mouse colon, C6, PC-12, rat brain, rat colon, COS-7纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:10000-1:100000 Hu, Ms, Rt, Mk IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu, Ms
Beta-actin is a fundamental component of the microfilament system within the cytoskeleton and is a highly conserved member of the actin protein family. Present in almost all eukaryotic cells, it exists in dynamic equilibrium between its monomeric (G-actin) and polymeric (F-actin) states. By polymerizing into helical microfilaments, it provides mechanical support and builds a network that governs intracellular transport, cell migration, division, and morphological maintenance. As a representative cytoplasmic actin, beta-actin not only mediates cell-matrix adhesion (e.g., focal adhesions) and cell-cell connections but also plays unconventional roles in transcriptional regulation and signal transduction by interacting with various proteins. Due to its extremely high and consistent expression levels across most tissues and cell types, the gene encoding beta-actin (ACTB) is widely used as a loading control or housekeeping gene reference in molecular biology experiments such as Western blot and qRT-PCR.
免疫印迹
WB result of Beta Actin Recombinant Mouse mAb
Primary antibody: Beta Actin Recombinant Mouse mAb at 1/40000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDaWB result of Beta Actin Recombinant Mouse mAb
Primary antibody: Beta Actin Recombinant Mouse mAb at 1/40000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: 4T1 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Lane 4: mouse colon lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDaWB result of Beta Actin Recombinant Mouse mAb
Primary antibody: Beta Actin Recombinant Mouse mAb at 1/40000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Lane 3: rat brain lysate 20 µg
Lane 4: rat colon lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDaWB result of Beta Actin Recombinant Mouse mAb
Primary antibody: Beta Actin Recombinant Mouse mAb at 1/40000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human heart. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse heart. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Beta Actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- Beta Actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Beta Actin in tumor tissues.
Expression of Beta Actin in human tissues.
Expression of Beta Actin in mouse & rat tissues.
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