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Rabbit抗原名称
PRSS35分子别名
Inactive serine protease 35; C6orf158免疫原
Synthetic Peptide细胞定位
SecretedAccession
Q8N3Z0克隆号
S-3769-241抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
PC-3, mouse liver, rat liver纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu
PRSS35, also known as serine protease 35 or neurosin, is a member of the S1 family of chymotrypsin-like serine proteases that is predominantly expressed in the central nervous system, particularly within neurons and oligodendrocytes. This enzyme plays a critical role in various physiological processes, including the regulation of myelin maintenance, synaptic plasticity, and the processing of extracellular matrix proteins such as myelin basic protein (MBP) and proteolipid protein (PLP). Research suggests that PRSS35 is involved in the turnover of myelin components, thereby influencing nerve conduction velocity and overall neural integrity, while its dysregulation or overexpression has been implicated in pathological conditions such multiple sclerosis, Alzheimer’s disease, and certain types of cancer, where it may contribute to tissue remodeling, inflammation, and tumor progression through the degradation of specific substrates and modulation of cell signaling pathways.
免疫印迹
WB result of PRSS35 Recombinant Rabbit mAb
Primary antibody: PRSS35 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 60 kDaWB result of PRSS35 Recombinant Rabbit mAb
Primary antibody: PRSS35 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 60 kDaWB result of Recombinant Rabbit mAb
Primary antibody: PRSS35 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 60 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-PRSS35 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-PRSS35 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-PRSS35 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-PRSS35 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in PC-3 cells. Anti-PRSS35 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of PRSS35 in tumor tissues.
Expression of PRSS35 in human tissues.
Expression of PRSS35 in mouse & rat tissues.
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