热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 其他一抗

所有图片（1/1）

Phospho-c-Kit (Tyr721) Recombinant Rabbit mAb (S-3481-5)

Mast/stem cell growth factor receptor Kit,SCFR,Piebald trait protein (PBT),Proto-oncogene c-Kit,Tyrosine-protein kinase Kit,p145 c-kit,v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog,CD117,KIT

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6892
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-c-Kit (Tyr721)

  • 分子别名

    Mast/stem cell growth factor receptor Kit; SCFR; Piebald trait protein (PBT); Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117; KIT

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Cell membrane

  • Accession

    P10721

  • 克隆号

    S-3481-5

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu

  • 阳性样本

    NCI-H562 (starved overnight, then treated with 100 ng/ml hSCF for 5 minutes)

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu
背景介绍

  • Phospho-c-Kit (Tyr721) refers to the phosphorylated form of the c-Kit receptor tyrosine kinase at tyrosine residue 721 within its intracellular juxtamembrane domain. Upon binding of its ligand stem cell factor (SCF), c-Kit undergoes autophosphorylation, including at Tyr721, which serves as a critical docking site for the p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3K). This phosphorylation event initiates downstream PI3K/Akt signaling cascades, thereby regulating essential cellular processes such as survival, proliferation, differentiation, and migration in hematopoietic stem cells, melanocytes, mast cells, and germ cells. Aberrant phosphorylation at this site has been implicated in various pathological conditions, including certain gastrointestinal stromal tumors (GISTs) and myeloproliferative disorders.

  • 免疫印迹

    • WB result of Phospho-c-Kit (Tyr721) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody incubation conditions: overnight at 4°C
      Primary antibody: Phospho-c-Kit (Tyr721) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NCI-H562 whole cell lysate 20 µg
      Lane 2: NCI-H562 starved overnight, then treated with 100 ng/ml hSCF for 5 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 110 kDa
      Observed MW: 150 kDa

评论(0)