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Rabbit抗原名称
NPM1分子别名
Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; Npm1免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cytoskeleton, NucleusAccession
Q61937抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Ms, Rt, Mk阳性样本
NIH/3T3, 3T3-L1, C2C12, PC-12, COS-7纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Ms, Rt, Mk IHC-P 1:125-1:250 Ms, Rt
Nucleophosmin (NPM1), also known as B23 or numatrin, is a highly abundant, multifunctional phosphoprotein primarily localized in the granular component of the nucleolus, where it plays pivotal roles in ribosome biogenesis, centrosome duplication, and the regulation of genomic stability. As a molecular chaperone with histone-binding activity, NPM1 facilitates the assembly and transport of ribosomal subunits from the nucleolus to the cytoplasm, thereby ensuring efficient protein synthesis, while also participating in the stress response by shuttling between the nucleus and cytoplasm to regulate the stability and activity of key tumor suppressors such as p53 and ARF. Structurally characterized by its ability to form pentameric rings and undergo liquid-liquid phase separation, NPM1 is crucial for maintaining nucleolar architecture; however, its dysfunction, particularly through cytoplasmic mislocalization caused by specific mutations like NPM1c+, is a defining driver event in acute myeloid leukemia (AML), highlighting its dual significance as both an essential housekeeping protein and a critical oncoprotein when deregulated.
免疫印迹
WB result of NPM1 Rabbit pAb
Primary antibody: NPM1 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: 3T3-L1 whole cell lysate 20 µg
Lane 3: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 38 kDaWB result of NPM1 Rabbit pAb
Primary antibody: NPM1 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 38 kDaWB result of NPM1 Rabbit pAb
Primary antibody: NPM1 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 38 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-NPM1 antibody was used at 1/125 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-NPM1 antibody was used at 1/125 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-NPM1 antibody was used at 1/125 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-NPM1 antibody was used at 1/125 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of NPM1 in mouse & rat tissues.
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