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Rabbit抗原名称
MCL1分子别名
Induced myeloid leukemia cell differentiation protein Mcl-1 homolog; Bcl-2-related protein EAT/mcl1; Mcl1免疫原
Synthetic Peptide细胞定位
Mitochondrion, Cytoplasm, NucleusAccession
P97287克隆号
S-3887-64抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
L929, NIH/3T3, C2C12, mouse spleen, C6, rat spleen纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt ICC 1:500 Ms
MCL1 (Myeloid cell leukemia 1) is an anti-apoptotic member of the BCL-2 protein family that plays a crucial role in regulating cell survival and death by binding to and neutralizing pro-apoptotic proteins such as BAK and BIM, thereby preventing mitochondrial outer membrane permeabilization and subsequent activation of the intrinsic apoptotic pathway.
免疫印迹
WB result of Mcl-1 Recombinant Rabbit mAb
Primary antibody: Mcl-1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: L-929 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: C2C12 whole cell lysate 20 µg
Lane 4: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 36, 38 kDaWB result of Mcl-1 Recombinant Rabbit mAb
Primary antibody: Mcl-1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 36, 38 kDa
免疫细胞化学
ICC shows positive staining in NIH/3T3 cells. Anti- Mcl-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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