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Rabbit抗原名称
CHX10分子别名
Visual system homeobox 2; Ceh-10 homeodomain-containing homolog; Homeobox protein CHX10; Chx10; Vsx2免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q61412克隆号
S-3940-13抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
IF ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:500 Hu, Ms, Rt ICC 1:500 Ms, Rt
CHX10, also known as VSX2 (Visual System Homeobox 2), is a paired-like homeodomain transcription factor that serves as a master regulator in vertebrate eye development, playing indispensable roles in the specification, proliferation, and differentiation of retinal progenitor cells into various neuronal lineages, particularly bipolar cells and Müller glia. Expressed primarily in the developing neural retina, CHX10 functions by binding to specific DNA sequences to activate or repress target genes involved in cell cycle control and fate determination, thereby ensuring the proper formation of retinal layers and overall eye morphology. Mutations in the CHX10 gene are associated with severe congenital eye disorders in both humans and animals, such as microphthalmia (abnormally small eyes) and anophthalmia (absence of eyes), highlighting its critical importance in oculogenesis; furthermore, its conserved expression pattern across species underscores its fundamental evolutionary role in establishing the visual system, making it a key marker for studying retinal development and a potential target for regenerative therapies aimed at restoring vision through stem cell-derived retinal tissues.
免疫组化
IHC shows positive staining in paraffin-embedded human retina. Anti-CHX10 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse retina. Anti-CHX10 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat retina. Anti-CHX10 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse retina. Anti-CHX10 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat retina. Anti-CHX10 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of CHX10 in human tissues.
Expression of CHX10 in mouse & rat tissues.
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