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宿主来源
Rabbit抗原名称
Phospho-Acetyl-CoA Carboxylase (Ser79)分子别名
Acetyl-CoA carboxylase 1; ACC1; Acetyl-Coenzyme A carboxylase alpha (ACC-alpha); ACAC; ACCA; ACACA细胞定位
CytoplasmAccession
Q13085克隆号
S-4302抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
MCF7 treated with 0.5 µM oligomycin for 30 minutes, 293T treated with 0.5 µM oligomycin for 30 minutes纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:250-1:1000 Hu IHC-P 1:250 Hu
Phospho-Acetyl-CoA Carboxylase (Ser79) refers to the specific phosphorylated state of Acetyl-CoA Carboxylase 1 (ACC1) at serine residue 79, a critical post-translational modification that serves as a key regulatory switch in lipid metabolism. This phosphorylation is primarily catalyzed by AMP-activated protein kinase (AMPK) in response to cellular energy stress or low ATP levels, leading to the allosteric inhibition of ACC enzymatic activity. By inhibiting ACC, the conversion of acetyl-CoA to malonyl-CoA is blocked, which subsequently reduces de novo fatty acid synthesis and relieves the inhibition of carnitine palmitoyltransferase 1 (CPT1), thereby promoting mitochondrial fatty acid oxidation. Consequently, the detection of ACC phosphorylated at Ser79 is widely used as a biomarker for AMPK activation and the metabolic shift from anabolic lipid storage to catabolic energy production, playing a significant role in the physiological responses to exercise, fasting, and pharmacological interventions targeting metabolic disorders.
免疫印迹
WB result of Phospho-Acetyl-CoA Carboxylase (Ser79) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-Acetyl-CoA Carboxylase (Ser79) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 treated with 0.5 µM oligomycin for 30 minutes whole cell lysate 20 µg
Lane 2: 293T treated with 0.5 µM oligomycin for 30 minutes whole cell lysate 20 µg
Lane 3: MCF7 treated with 0.5 µM oligomycin for 30 minutes whole cell lysate 20 µg (phosphatase treated membrane)
Lane 4: 293T treated with 0.5 µM oligomycin for 30 minutes whole cell lysate 20 µg (phosphatase treated membrane)
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 265 kDa
Observed MW: 270 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Phospho-Acetyl-CoA Carboxylase (Ser79) antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in λ-PPase treated paraffin-embedded human colon cancer. Anti- Phospho-Acetyl-CoA Carboxylase (Ser79) antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Phospho-Acetyl-CoA Carboxylase (Ser79) in tumor tissues.
Expression of Phospho-Acetyl-CoA Carboxylase (Ser79) in human tissues.
Expression of Phospho-Acetyl-CoA Carboxylase (Ser79) in mouse & rat tissues.
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