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宿主来源
Rabbit抗原名称
STEAP1+STEAP1B1+SPEAP1B2分子别名
STEAP1 protein; PRSS24; STEAP细胞定位
Endosome, Cell membraneAccession
Q9UHE8克隆号
S-3226抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
LNCap, 22Rv1纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:2000 Hu
STEAP1, STEAP1B1, and STEAP1B2 (also known as STEAP1B) belong to the STEAP (Six-Transmembrane Epithelial Antigen of the Prostate) protein family. A common feature of these proteins is that they all contain a C-terminal domain homologous to yeast ferric reductase (FRE), which includes a b-type heme prosthetic group—a core component essential for their potential metal reductase function. Among them, STEAP1 is the most extensively studied member of the family. It is highly upregulated on the cell surface of various human cancers (such as prostate cancer and lung cancer) and is therefore regarded as a highly promising target for cancer therapy. However, unlike other family members (STEAP2–4), STEAP1 lacks an intracellular NADPH-binding domain and thus does not possess independent ferric reductase activity. Cryo-electron microscopy structures have revealed that STEAP1 exists as a trimer, and it is hypothesized that it functions by forming heterotrimeric complexes with other family members such as STEAP2 or STEAP4, which provide the NADPH-binding domain, thereby cooperating to complete the reduction of metal ions. Meanwhile, STEAP1B1 and STEAP1B2, as homologs (or truncated homologs) of STEAP1, also possess the ferric reductase-like transmembrane domain. However, their specific biological functions, tissue distribution, and precise mechanisms of interaction with STEAP1 remain to be further elucidated. Current studies suggest that they may have synergistic or compensatory functional relationships with STEAP1.
免疫印迹
WB result of STEAP1+STEAP1B1+SPEAP1B2 Recombinant Rabbit mAb
Primary antibody: STEAP1+STEAP1B1+SPEAP1B2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: DU 145 whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Lane 4: 22Rv1 whole cell lysate 20 µg
Negative control: PC-3 whole cell lysate; DU 145 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 35 kDaWB result of STEAP1+STEAP1B1+SPEAP1B2 Recombinant Rabbit mAb
Primary antibody: STEAP1+STEAP1B1+SPEAP1B2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293F transfected with human STEAP2 expression vector containing a His-tag whole cell lysate 20 µg
Lane 2: 293F transfected with human STEAP3 expression vector containing a His-tag whole cell lysate 20 µg
Lane 3: 293F transfected with human STEAP4 expression vector containing a His-tag whole cell lysate 20 µg
Lane 4: 293F transfected with human STEAP1B1 expression vector containing a His-tag whole cell lysate 20 µg
Lane 5: 293F transfected with human STEAP1B2 expression vector containing a His-tag whole cell lysate 20 µg
Lane 6: 293F transfected with human STEAP1 expression vector containing a His-tag whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 35 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human prostatic cancer (case 1). Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer (case 2). Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer (case 3). Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer (case 4). Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human endometrial cancer. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human STEAP1 transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human STEAP1B1 transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human STEAP1B2 transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human STEAP2 transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human STEAP3 transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human STEAP4 transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded vector transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of STEAP1+STEAP1B1+STEAP1B2 in prostate tumor tissues.
Expression of STEAP1+STEAP1B1+STEAP1B2 in tumor tissues.
Expression of STEAP1+STEAP1B1+STEAP1B2 in human tissues.
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