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宿主来源
Rabbit抗原名称
STEAP1分子别名
STEAP1 protein; PRSS24; STEAP免疫原
Synthetic Peptide细胞定位
Cell membrane, EndosomeAccession
Q9UHE8克隆号
S-3613-107抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
LNCap, 22Rv1纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu IHC-P 1:2000 Hu
STEAP1 (Six-Transmembrane Epithelial Antigen of the Prostate 1) is a metalloreductase protein that plays a crucial role in cellular iron and copper homeostasis by facilitating the reduction of Fe³⁺ to Fe²⁺ and Cu²⁺ to Cu⁺, thereby enabling the transport of these essential metal ions across cell membranes. Primarily expressed in prostate epithelium, STEAP1 has gained significant attention as a promising therapeutic target in oncology, particularly for prostate cancer, where it is frequently overexpressed and serves as a cell surface antigen for antibody-drug conjugates and CAR-T cell therapies.
免疫印迹
WB result of STEAP1 Recombinant Rabbit mAb
Primary antibody: STEAP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: DU 145 whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Lane 4: 22Rv1 whole cell lysate 20 µg
Negative control: PC-3 whole cell lysate; DU 145 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 35 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human STEAP1 transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human STEAP1B1 transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human STEAP1B2 transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human STEAP2 transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human STEAP3 transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human STEAP4 transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded vector transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded PC-3 cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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