Phospho-eNOS (Ser1177) Recombinant Rabbit mAb (S-3448)

Phospho-eNOS (Ser1177) refers to endothelial nitric oxide synthase (eNOS) that has been phosphorylated at the serine 1177 residue, a critical post-translational modification that serves as a primary regulatory mechanism for enhancing the enzyme's catalytic activity and nitric oxide (NO) production in vascular endothelial cells. This specific phosphorylation event, predominantly mediated by kinases such as Akt (Protein Kinase B), AMP-activated protein kinase (AMPK), and protein kinase A (PKA) in response to physiological stimuli like shear stress, growth factors, or insulin, induces a conformational change that increases electron flux from the reductase domain to the oxygenase domain while simultaneously reducing the enzyme's dependence on intracellular calcium levels. Consequently, the activation of eNOS via Ser1177 phosphorylation leads to sustained NO release, which is essential for maintaining vascular homeostasis by promoting vasodilation, inhibiting platelet aggregation, preventing leukocyte adhesion, and exerting anti-atherogenic effects, thereby making this modified protein form a key biomarker and therapeutic target in cardiovascular research related to hypertension, atherosclerosis, and endothelial dysfunction.