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宿主来源
Rabbit抗原名称
Phospho-RIP3 (Ser227)分子别名
Receptor-interacting serine/threonine-protein kinase 3; RIP-like protein kinase 3; Receptor-interacting protein 3 (RIP-3); RIP3; RIPK3细胞定位
Nucleus, CytoplasmAccession
Q9Y572克隆号
S-3488抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HT-29 (treated with 20 μM Z-VAD for 30 minutes, then 20 ng/ml hTNF-α and 100 nM SM-164 were added for 7 hours)纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
Phospho-RIP3 (Ser227) refers to the phosphorylated form of Receptor-Interacting Protein Kinase 3 (RIP3) at serine residue 227, a critical post-translational modification that plays a pivotal role in regulating necroptosis—a programmed form of necrotic cell death. This specific phosphorylation event, typically mediated by upstream kinases such as RIP1 or other stimuli, serves as a molecular switch that activates RIP3's kinase activity and promotes its interaction with downstream effectors like MLKL (mixed lineage kinase domain-like protein). The phosphorylation at Ser227 is essential for the assembly of the necrosome complex, propagation of death signals, and execution of necroptotic cell death, making it a key biomarker for studying inflammatory responses, tissue injury, and various pathological conditions including neurodegenerative diseases and ischemia-reperfusion injury.
免疫印迹
WB result of Phospho-RIP3 (Ser227) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-RIP3 (Ser227) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HT-29 whole cell lysate 20 µg
Lane 2: HT-29 treated with 20 μM Z-VAD for 30 minutes, then 20 ng/ml hTNF-α and 100 nM SM-164 were added for 7 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 62 kDa
This blot was developed with high sensitivity substrate
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