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Rabbit抗原名称
Phospho-DRP1 (Ser616)分子别名
Density-regulated protein; Protein DRP1; Smooth muscle cell-associated protein 3 (SMAP-3); DRP1; DENR细胞定位
CytoplasmAccession
O43583克隆号
S-4221抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HeLa (treated with 100 ng/ml Calyculin A for 30 minutes), HeLa (treated with 100 ng/ml Nocodazole for 17 hours), NIH/3T3 (treated with 100 ng/ml Calyculin A for 30 minutes)纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms
Phospho-DRP1 (Ser616) denotes the phosphorylation of Dynamin-related protein 1 (DRP1) at serine residue 616, a critical post-translational modification that promotes mitochondrial fission. This specific phosphorylation event is primarily mediated by kinases such as Cyclin-dependent kinase 1 (CDK1/Cyclin B) during mitosis, as well as by extracellular signal-regulated kinase 1/2 (ERK1/2) and Protein Kinase C delta (PKCδ) in response to various cellular stresses or growth signals. Phosphorylation at Ser616 enhances the GTPase activity of DRP1 and facilitates its translocation from the cytosol to the outer mitochondrial membrane, where it oligomerizes to constrict and divide mitochondria. This process is essential for maintaining mitochondrial dynamics, ensuring equal distribution of mitochondria during cell division, and regulating apoptosis; conversely, dysregulation of this site is often associated with pathological conditions involving excessive mitochondrial fragmentation, such as neurodegenerative diseases and cardiac injury.
免疫印迹
WB result of Phospho-DRP1 (Ser616) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-DRP1 (Ser616) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Lane 3: untreated HeLa whole cell lysate 20 µg
Lane 4: HeLa treated with 100 ng/ml Nocodazole for 17 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrateWB result of Phospho-DRP1 (Ser616) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-DRP1 (Ser616) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 80 kDa
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