分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
CENP-F分子别名
Centromere protein F; AH antigen; Kinetochore protein CENPF; Mitosin; CENPF免疫原
Recombinant Protein细胞定位
Nucleus, Cytoplasm, CytoskeletonAccession
P49454克隆号
S-3148-75抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:500 Hu
CENP-F (Centromere Protein F), also known as mitosin, is a large, transient kinetochore protein that plays a critical role in ensuring accurate chromosome segregation during cell division. Primarily localized to the kinetochores from late G2 phase through metaphase, CENP-F functions as a key scaffold and regulatory hub that facilitates the attachment of spindle microtubules to chromosomes. It interacts with various components of the mitotic machinery, including dynein and the nuclear pore complex protein Nup133, to coordinate spindle assembly checkpoint signaling and maintain genomic stability. Due to its essential function in mitosis and its frequent overexpression in various malignancies, CENP-F is often studied as a potential biomarker for cancer prognosis and a target for anti-mitotic therapies.
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-CENP-F antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-CENP-F antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CENP-F antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CENP-F antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-CENP-F antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CENP-F antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CENP-E in tumor tissues.
Expression of CENP-E in human tissues.
评论(0)