Phospho-HSP27 (Ser82) Recombinant Rabbit mAb (S-4792)
Heat shock protein beta-1,28 kDa heat shock protein,Estrogen-regulated 24 kDa protein,Heat shock 27 kDa protein (HSP 27),Heat shock protein family B member 1,Stress-responsive protein 27 (SRP27),HSP27,HSP28,HSPB1
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宿主来源
Rabbit抗原名称
Phospho-HSP27 (Ser82)分子别名
Heat shock protein beta-1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein (HSP 27); Heat shock protein family B member 1; Stress-responsive protein 27 (SRP27); HSP27; HSP28; HSPB1细胞定位
Cytoplasm, Cytoskeleton, NucleusAccession
P04792克隆号
S-4792抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HeLa treated with 25 μg/ml Anisomycin for 30 minutes, C2C12 treated with 25 μg/ml Anisomycin for 30 minutes纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:100 Hu ICC 1:50-1:100 Hu, Ms
Phospho-HSP27 (Ser82) refers to the heat shock protein 27 (HSP27, also known as HSPB1) specifically phosphorylated at the serine 82 residue, a critical post-translational modification primarily mediated by the MAPKAP kinase 2 (MK2) downstream of the p38 MAPK signaling pathway in response to cellular stressors such as heat shock, oxidative stress, or inflammatory cytokines. This specific phosphorylation event induces a conformational change that causes the dissociation of large HSP27 oligomers into smaller dimers or monomers, thereby enhancing its chaperone activity and ability to interact with client proteins, while simultaneously playing a pivotal role in stabilizing the actin cytoskeleton, inhibiting apoptosis by preventing cytochrome c release and caspase activation, and promoting cell survival and proliferation. Due to its significant upregulation and activation in various malignancies, Phospho-HSP27 (Ser82) is widely recognized not only as a key regulator of cellular stress responses and cytoprotection but also as a potential biomarker for cancer prognosis and a therapeutic target in diseases characterized by dysregulated stress signaling pathways.
免疫印迹
WB result of Phospho-HSP27 (Ser82) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-HSP27 (Ser82) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 25 μg/ml Anisomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 25 kDaWB result of Phospho-HSP27 (Ser82) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-HSP27 (Ser82) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C2C12 whole cell lysate 20 µg
Lane 2: C2C12 treated with 25 μg/ml Anisomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 25 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- Phospho-HSP27 (Ser82) antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in lambda phosphatase treated paraffin-embedded human cervical squamous cell carcinoma. Anti- Phospho-HSP27 (Ser82) antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Phospho-HSP27 (Ser82) antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in lambda phosphatase treated paraffin-embedded human colon cancer. Anti- Phospho-HSP27 (Ser82) antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti- Phospho-HSP27 (Ser82) antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in lambda phosphatase treated paraffin-embedded human tonsil. Anti- Phospho-HSP27 (Ser82) antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC analysis of Hela cells treated with λ phosphatase (top panel) and untreated Hela cells (below panel). Anti- Phospho-HSP27 (Ser82) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC analysis of C2C12 cells treated with λ phosphatase (top panel) and untreated C2C12 cells (below panel). Anti- Phospho-HSP27 (Ser82) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Phospho-HSP27 (Ser82) in tumor tissues.
Expression of Phospho-HSP27 (Ser82) in human tissues.
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