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宿主来源
Rabbit抗原名称
Phospho-CSF-1R/M-CSF-R (Tyr699)分子别名
Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor (CSF-1-R; CSF-1R; M-CSF-R); Proto-oncogene c-Fms; CD115; FMS; CSF1R细胞定位
Cell membraneAccession
P07333克隆号
S-3553抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
THP-1 starve overnight, then treated with 100 ng/ml hM-CSF for 5 minutes纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
Phospho-CSF-1R/M-CSF-R (Tyr699) specifically refers to the colony-stimulating factor 1 receptor (M-CSF-R, encoded by the proto-oncogene c-fms) phosphorylated at tyrosine residue 699. Located within the kinase insert domain of the receptor, Tyr699 undergoes autophosphorylation upon ligand-induced dimerization (e.g., with M-CSF or IL-34). This phosphorylation event creates a critical docking site for the adaptor protein Grb2, thereby initiating downstream signaling pathways including PI3K/Akt and ERK, which are essential for regulating the survival, proliferation, and differentiation of mononuclear phagocytes such as macrophages and microglia. Consequently, detecting the phosphorylation status of Tyr699 serves as a key indicator of CSF-1R activation and holds significant research value in areas such as cancer (e.g., ovarian and breast cancer) and neurological disorders (e.g., adult-onset leukoencephalopathy).
免疫印迹
WB result of Phospho-CSF-1R/M-CSF-R (Tyr699) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-CSF-1R/M-CSF-R (Tyr699) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 unboiled whole cell lysate 20 µg
Lane 2: THP-1 starve overnight, then treated with 100 ng/ml hM-CSF for 5 minutes unboiled whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
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