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宿主来源
Rabbit抗原名称
CD134分子别名
Tumor necrosis factor receptor superfamily member 4; OX40 antigen; OX40L receptor; Ox40; Txgp1; Tnfrsf4细胞定位
MembraneAccession
P47741克隆号
S-4797抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse splenocytes treated with 2.5 μg/ml concanavalin A for 72 hours, mouse spleen, rat spleen纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:100 Ms, Rt
CD134, also known as OX40 or TNFRSF4, is a costimulatory molecule belonging to the tumor necrosis factor receptor (TNFR) superfamily that is primarily expressed on the surface of activated CD4+ and CD8+ T cells, playing a pivotal role in regulating adaptive immune responses by binding to its ligand, OX40L (CD252), which is typically found on antigen-presenting cells such as dendritic cells, B cells, and macrophages. This interaction delivers a critical secondary signal that enhances T cell proliferation, survival, and cytokine production, particularly by upregulating anti-apoptotic proteins like Bcl-2 and Bcl-xL, thereby preventing activation-induced cell death and promoting the formation of long-lived memory T cells. Due to its significant influence on sustaining T cell activity, CD134 has emerged as a major target in cancer immunotherapy, where agonistic antibodies are designed to boost anti-tumor immunity, while blocking antibodies are explored for treating autoimmune diseases by dampening excessive immune responses, highlighting its dual potential in both enhancing and suppressing immune pathways depending on the clinical context.
免疫印迹
WB result of CD134 Recombinant Rabbit mAb
Primary antibody: CD134 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated mouse splenocytes whole cell lysate 20 µg
Lane 2: mouse splenocytes treated with 2.5 μg/ml concanavalin A for 72 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 47 kDaWB result of CD134 Recombinant Rabbit mAb
Primary antibody: CD134 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Negative control: mouse brain lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 47 kDaWB result of CD134 Recombinant Rabbit mAb
Primary antibody: CD134 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 47 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD134 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-CD134 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CD134 in mouse & rat tissues.
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