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Calreticulin Recombinant Rabbit mAb (S-3920-39)

CRP55,Calregulin,Endoplasmic reticulum resident protein 60 (ERp60),HACBP,grp60,CRTC,CALR

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6841
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Calreticulin

  • 分子别名

    CRP55; Calregulin; Endoplasmic reticulum resident protein 60 (ERp60); HACBP; grp60; CRTC; CALR

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Endoplasmic reticulum lumen

  • Accession

    P27797

  • 克隆号

    S-3920-39

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, HL-60, HepG2, NIH/3T3, RAW264.7, Neuro-2a, C2C12, mouse heart, mouse brain, mouse colon, mouse stomach, mouse kidney, mouse liver, rat heart, rat brain, rat colon, rat stomach, rat kidney, rat liver

  • 纯化方式

    Protein A

  • 浓度

    0.25 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:5000 Hu, Ms, Rt
    IHC-P 1:2500 Hu, Ms, Rt
    ICC 1:250 Ms
背景介绍

  • Calreticulin (CRT) is a highly conserved, multifunctional calcium-binding chaperone protein primarily residing in the lumen of the endoplasmic reticulumER), where it plays a pivotal role in maintaining cellular calcium homeostasis and facilitating the proper folding and quality control of newly synthesized glycopptides through its interaction with the calnexin/calreticulin cycle. Beyond its canonical intracellular functions, CRT exhibits significant "moonlighting" properties by translocating to the cell surface under stress conditions, such as immunogenic cell death, where it acts as a critical "eat-me" signal that promotes the phagocytosis of apoptotic or dying cells by dendritic cells and macrophages via binding to the LDL receptor-related protein 1 (LRP1/CD91), thereby bridging innate and adaptive immunity. Furthermore, extracellular CRT has been implicated in various pathological processes, including wound healing, angiogenesis, and autoimmune diseases like systemic lupus erythematosus, making it a subject of intense research for potential therapeutic interventions in cancer immunotherapy and inflammatory disorders.

  • 免疫印迹

    • WB result of Calreticulin Recombinant Rabbit mAb
      Primary antibody: Calreticulin Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: HL-60 whole cell lysate 20 µg
      Lane 3: HepG2 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48 kDa
      Observed MW: 58 kDa

    • WB result of Calreticulin Recombinant Rabbit mAb
      Primary antibody: Calreticulin Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: RAW264.7 whole cell lysate 20 µg
      Lane 3: Neuro-2a whole cell lysate 20 µg
      Lane 4: C2C12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48 kDa
      Observed MW: 58 kDa

    • WB result of Calreticulin Recombinant Rabbit mAb
      Primary antibody: Calreticulin Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse heart lysate 20 µg
      Lane 2: mouse brain lysate 20 µg
      Lane 3: mouse colon lysate 20 µg
      Lane 4: mouse stomach lysate 20 µg
      Lane 5: mouse kidney lysate 20 µg
      Lane 6: mouse liver lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48 kDa
      Observed MW: 58 kDa

    • WB result of Calreticulin Recombinant Rabbit mAb
      Primary antibody: Calreticulin Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat heart lysate 20 µg
      Lane 2: rat brain lysate 20 µg
      Lane 3: rat colon lysate 20 µg
      Lane 4: rat stomach lysate 20 µg
      Lane 5: rat kidney lysate 20 µg
      Lane 6: rat liver lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48 kDa
      Observed MW: 58 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in NIH/3T3 cells. Anti-Calreticulin antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Calreticulin in tumor tissues.

    • Expression of Calreticulin in human tissues.

    • Expression of Calreticulin in mouse & rat tissues.

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