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Rabbit抗原名称
CYTB分子别名
Cystatin-B; CPI-B; Liver thiol proteinase inhibitor; Stefin-B; CST6; STFB; CSTB免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P04080克隆号
S-3877-15抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt, Mk IHC-P 1:200 Hu, Ms, Rt ICC 1:500 Ms
Cytochrome b (CYTB) is a crucial transmembrane protein encoded by the mitochondrial DNA in most eukaryotes, serving as the central catalytic subunit of Complex III (the cytochrome bc1 complex) within the electron transport chain. This protein contains two heme groups, heme bL and heme bH, which facilitate the transfer of electrons from ubiquinol to cytochrome c while simultaneously pumping protons across the inner mitochondrial membrane to generate the electrochemical gradient necessary for ATP synthesis via oxidative phosphorylation. Due to its essential role in cellular respiration, CYTB is highly conserved across species yet accumulates mutations at a rate suitable for phylogenetic studies, making it a standard molecular marker for evolutionary biology, species identification, and forensic analysis, while specific mutations in the human CYTB gene are also linked to mitochondrial diseases affecting energy-intensive tissues such as muscle and the nervous system.
免疫印迹
WB result of CYTB Recombinant Rabbit mAb
Primary antibody: CYTB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: U-2 OS whole cell lysate 20 µg
Lane 3: MDA-MB-231 whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 43 kDaWB result of CYTB Recombinant Rabbit mAb
Primary antibody: CYTB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 43 kDaWB result of CYTB Recombinant Rabbit mAb
Primary antibody: CYTB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 43 kDaWB result of CYTB Recombinant Rabbit mAb
Primary antibody: CYTB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 43 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-CYTB antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CYTB antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-CYTB antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-CYTB antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-CYTB antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in C2C12 cells. Anti-CYTB antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of CYTB in tumor tissues.
Expression of CYTB in human tissues.
Expression of CYTB in mouse & rat tissues.
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